TRIM27 Antibody (PCRP-TRIM27-1B3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07415

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-TRIM27-1B3

Concentration

0.2 mg/ml

View all TRIM27 Antibodies »

Product Summary for TRIM27 Antibody (PCRP-TRIM27-1B3)

Immunogen

Recombinant full-length human TRIM27 protein (Uniprot: P14373)

Reactivity Notes

Predicted to react with Mouse and Rat.

Localization

Nucleus, Cytoplasm. Nucleus > PML body. Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

58.49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08579)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TRIM27 Antibody (PCRP-TRIM27-1B3)

Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Immunocytochemistry/Immunofluorescence: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Immunofluorescence Analysis of PFA-fixed U87 cells using TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Flow Cytometry: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Flow Cytometry: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Protein Array: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415]

Protein Array: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3).

Applications for TRIM27 Antibody (PCRP-TRIM27-1B3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRIM27

The TRIM27 gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the

Alternate Names

Ret finger proteinRING finger protein 76, RFPtripartite motif protein TRIM27, RNF76RFP transforming protein, tripartite motif containing 27, tripartite motif-containing 27, Tripartite motif-containing protein 27, zinc finger protein RFP

Gene Symbol

TRIM27

UniProt

P14373 (Human)

Additional TRIM27 Products

Product Documents for TRIM27 Antibody (PCRP-TRIM27-1B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM27 Antibody (PCRP-TRIM27-1B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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