TRIM72 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52104

Novus Biologicals, part of Bio-Techne
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NBP1-52104
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TRIM72 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RPQALSTNLQ corresponding to internal region according to NP_001008275.1.

Epitope

C-RPQALSTNLQ

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM72 Antibody

Western Blot: TRIM72 Antibody [NBP1-52104]

Western Blot: TRIM72 Antibody [NBP1-52104]

Western Blot: TRIM72 Antibody [NBP1-52104] - Staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM72 Antibody [NBP1-52104]

Immunohistochemistry-Paraffin: TRIM72 Antibody [NBP1-52104]

Immunohistochemistry-Paraffin: TRIM72 Antibody [NBP1-52104] - Staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TRIM72 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 52 kDa band observed in mouse heart lysates (calculated MW of 52.8 kDa band according to mouse NP_001073401.1). IHC: Human skeletal muscle shows staining of the fibre membranes, detached artificially by the sectioning.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM72

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of therepair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membranedamage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization atthe injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to theinjury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealingprocess. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membranebudding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (Bysimilarity)

Alternate Names

MG53Mg53, Mitsugumin-53, tripartite motif containing 72, tripartite motif-containing 72, tripartite motif-containing protein 72

Entrez Gene IDs

493829 (Human); 434246 (Mouse); 365377 (Rat)

Gene Symbol

TRIM72

UniProt

NP_001008275.1 (Human)

Additional TRIM72 Products

Product Documents for TRIM72 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRIM72 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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