TRIO Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32088
![Western Blot: TRIO Antibody [NBP2-32088]](https://resources.bio-techne.com/images/products/TRIO-Antibody-Western-Blot-NBP2-32088-img0002.jpg "Western Blot: TRIO Antibody [NBP2-32088]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1575 to 1625 of human Trio Rho Guanine Nucleotide Exchange Factor using the numbering given in entry NP_009049.2 (GeneID 7204).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRIO Antibody - BSA Free
Western Blot: TRIO Antibody [NBP2-32088]
Western Blot: TRIO Antibody [NBP2-32088] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIO antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: TRIO Antibody [NBP2-32088]](https://resources.bio-techne.com/images/products/TRIO-Antibody-Immunoprecipitation-NBP2-32088-img0003.jpg "Immunoprecipitation: TRIO Antibody [NBP2-32088]")
Immunoprecipitation: TRIO Antibody [NBP2-32088]
Immunoprecipitation: TRIO Antibody [NBP2-32088] - Detection of human TRIO by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIO antibody NBP2-32088 used for IP at 3 ug per reaction. TRIO was also immunoprecipitated by 2 other rabbit anti-TRIO antibodies. For blotting immunoprecipitated TRIO, NBP2-32088 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for TRIO Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 3-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TRIO
Alternate Names
ARHGEF23, EC 2.7.11.1, FLJ42780, PTPRF-interacting protein, tgat, triple functional domain (PTPRF interacting), triple functional domain protein
Gene Symbol
TRIO
UniProt
Additional TRIO Products
Product Documents for TRIO Antibody - BSA Free
Product Specific Notices for TRIO Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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