Triosephosphate isomerase Antibody (181)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43761

Novus Biologicals, part of Bio-Techne
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NBP2-43761
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 181

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Triosephosphate isomerase Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Triosephosphate isomerase Antibody (181)

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [181] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [181] diluted at 1:200. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Mouse testis. Triosephosphate isomerase stained by Triosephosphate isomerase antibody [181] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Triosephosphate isomerase Antibody (181)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Gene Symbol

TPI1

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody (181)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Triosephosphate isomerase Antibody (181)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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