Triosephosphate isomerase Antibody (1D10-2E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00007167-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D10-2E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Triosephosphate isomerase Antibodies »

Product Specifications

Immunogen

TPI1 (AAH07086.1, 1 a.a. ~ 249 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDTINAKQ

Localization

Cytoplasmic

Specificity

TPI1 - triosephosphate isomerase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Triosephosphate isomerase Antibody (1D10-2E2)

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01]

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01]

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - TPI1 monoclonal antibody (M01), clone 1D10-2E2 Analysis of TPI1 expression in HepG2.
Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01]

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01]

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Antibody Reactive Against Recombinant TPI1 (AAH07086, 1 a.a. ~ 250 a.a) ) full length protein with GST tag (53.5 KDa) .
Sandwich ELISA: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Detection limit for recombinant GST tagged FLJ23834 is approximately 0.1ng/ml as a capture antibody.
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Applications for Triosephosphate isomerase Antibody (1D10-2E2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Entrez Gene IDs

7167 (Human)

Gene Symbol

TPI1

OMIM

190450 (Human)

UniProt

AAH07086 (Human)

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody (1D10-2E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Triosephosphate isomerase Antibody (1D10-2E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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