TrkB [p Tyr816] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03499
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide from the region around (and including) phospho-Tyr816 site of rat TrkB conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in human and mouse.
Modification
p Tyr816
Localization
subcellular location: membrane; single-pass type i membrane protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TrkB [p Tyr816] Antibody
![Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499]](https://resources.bio-techne.com/images/products/TrkB-[p-Tyr816]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03499-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499]")
Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499]
Immunocytochemistry/Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499] - Immunofluorescence double staining assay of BDNF and p-TrkB in the SDH. BDNF and p-TrkB (red), NeuN, GFAP, and OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. And TrkB receptors expressed in neurons, microglia, and astrocytes. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-1704-0), licensed under a CC-BY license.Applications for TrkB [p Tyr816] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
10-50 ug/ml
Immunohistochemistry-Frozen
10-50 ug/ml
Immunohistochemistry-Paraffin
10-50 ug/ml
Western Blot
10-50 ug/ml
Application Notes
This antibody has been used in IHC-Frozen as reported in scientific literature (PMID: 23432085), ICC/IF reported in scientific literature (PMID: 23658180).
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Reconstitution
Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.
Formulation
Lyophilized from PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TrkB
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
UniProt
Additional TrkB Products
Product Documents for TrkB [p Tyr816] Antibody
Product Specific Notices for TrkB [p Tyr816] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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