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Tropomodulin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86870

Novus Biologicals, part of Bio-Techne
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NBP1-86870

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEKEALEHKDREDYVPYTGEKKGKIFIPKQKPVQTFTEEKVSLDPELEEALTSASDTELCDLAAILGMHNLITNTKFCNIMGSSNGVDQEHFSNVVKGEKILPVFDEPPNPTNVEESLKRTKENDAHLVEVNLNNIKNIPIPTLKD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25486436)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tropomodulin 3 Antibody

Western Blot: Tropomodulin 3 Antibody [NBP1-86870]

Western Blot: Tropomodulin 3 Antibody [NBP1-86870]

Western Blot: Tropomodulin 3 Antibody [NBP1-86870] - Analysis in human cell lines A-549 and HEK293 using anti-TMOD3 antibody. Corresponding TMOD3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunocytochemistry/ Immunofluorescence: Tropomodulin 3 Antibody [NBP1-86870]

Immunocytochemistry/ Immunofluorescence: Tropomodulin 3 Antibody [NBP1-86870]

Immunocytochemistry/Immunofluorescence: Tropomodulin 3 Antibody [NBP1-86870] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tropomodulin 3 Antibody [NBP1-86870]

Immunohistochemistry-Paraffin: Tropomodulin 3 Antibody [NBP1-86870]

Immunohistochemistry-Paraffin: Tropomodulin 3 Antibody [NBP1-86870] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Applications for Tropomodulin 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tropomodulin 3

Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton

Alternate Names

tropomodulin 3 (ubiquitous), tropomodulin-3, Ubiquitous tropomodulin, UTMOD, U-Tmod

Gene Symbol

TMOD3

Additional Tropomodulin 3 Products

Product Documents for Tropomodulin 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tropomodulin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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