Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8H8

Concentration

1 mg/ml

View all Troponin I Type 1 (slow skeletal) Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TNNI1 (NP_003272) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Troponin I Type 1.
Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170] - Human colorectal cancer tissue section stained with Troponin I Type 1 (slow skeletal) Antibody (OTI8H8). Incubation O/N at 4C with antibody at 1:200. IHC-P image submitted by a verified customer review.
Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170]

Immunohistochemistry: Troponin I Type 1 (slow skeletal) Antibody (OTI8H8) [NBP2-46170] - Analysis of Human adult heart tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120C for 3 minutes.
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Applications for Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-46170 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I Type 1 (slow skeletal)

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Gene Symbol

TNNI1

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Troponin I Type 1 (slow skeletal) Antibody (OTI8H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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