Troponin I Type 1 (slow skeletal) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56641

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Troponin I Type 1 (slow skeletal) Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding toTroponin I Type 1 (slow skeletal). The peptide sequence was selected from the N terminal of TNNI1 (NP_003272). Peptide sequence MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIP. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMIDs: 23751911 and 23063798). Porcine reactivity reported in scientific literature (PMID: 25635834).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Troponin I Type 1 (slow skeletal) Antibody

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Western-blots for a selection of differentially-expressed proteins. MyH2b, Troponin I Type 1 (slow skeletal) (TNNI1) and MCK were used as target protein; GADPH was used as a control. Total protein from control and arginine-supplemented pig samples was fractionated by SDS-PAGE, blotted and then detected with specific antibodies. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117294), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human Skeletal Muscle, 5.0ug/ml.
Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml
View 2 more images

Applications for Troponin I Type 1 (slow skeletal) Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml
Application Notes
Use in ICC/IF was reported in the scientific literature (PMID: 23751911).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I Type 1 (slow skeletal)

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Entrez Gene IDs

21952 (Mouse); 7135 (Rat); 29388 (Rat)

Gene Symbol

TNNI1

UniProt

P19237 (Human)

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Troponin I Type 1 (slow skeletal) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin I Type 1 (slow skeletal) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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