Troponin T type 1 (slow skeletal) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26095

Novus Biologicals, part of Bio-Techne
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NBP2-26095
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Troponin T type 1 (slow skeletal) Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKAQELSDWIHQLE corresponding to internal region according to NP_003274.3, NP_001119604.1, NP_001119605.1.

Specificity

This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Troponin T type 1 (slow skeletal) Antibody

Western Blot: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095]

Western Blot: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095]

Western Blot: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095] - Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095]

Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095]

Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP2-26095] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Troponin T type 1 (slow skeletal) Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Single Cell Western

100 ug/mL

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx. 37+35 kDa band bands observed in human skeletal muscle lysates (calculated MW of 32.9 kDa band according to NP_003274.3). This molecular weight is routinely observed by other sources. SCW validated using iPSC-derived cardiomyocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin T type 1 (slow skeletal)

Troponin T type 1 encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ANMtroponin T, slow skeletal muscle, FLJ98147, MGC104241, Slow skeletal muscle troponin T, sTnT, TNTS, TNTtroponin T1, skeletal, slow, troponin T type 1 (skeletal, slow), troponin-T1, skeletal, slow

Entrez Gene IDs

7138 (Human)

Gene Symbol

TNNT1

Additional Troponin T type 1 (slow skeletal) Products

Product Documents for Troponin T type 1 (slow skeletal) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin T type 1 (slow skeletal) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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