Tryptophanyl tRNA synthetase Antibody (3B2F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16419

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16419-100ul
NBP3-16419-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B2F10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for Tryptophanyl tRNA synthetase Antibody (3B2F10)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3-98 of human Tryptophanyl tRNA synthetase (P23381). NSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tryptophanyl tRNA synthetase Antibody (3B2F10)

Western Blot: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419] - Western blot analysis of extracts of various cell lines, using Tryptophanyl tRNA synthetase Rabbit mAb (NBP3-16419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419] - Immunohistochemistry of paraffin-embedded human placenta using Tryptophanyl tRNA synthetase Rabbit mAb (NBP3-16419) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for Tryptophanyl tRNA synthetase Antibody (3B2F10)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tryptophanyl tRNA synthetase

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

EC 6.1.1, EC 6.1.1.2, GAMMA-2, hWRS, IFI53, IFP53trpRS, Interferon-induced protein 53, TrpRS, tryptophan tRNA ligase 1, cytoplasmic, Tryptophan--tRNA ligase, tryptophanyl-tRNA synthetase, tryptophanyl-tRNA synthetase, cytoplasmic, WRS

Gene Symbol

WARS1

Additional Tryptophanyl tRNA synthetase Products

Product Documents for Tryptophanyl tRNA synthetase Antibody (3B2F10)

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Certificate of Analysis

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Product Specific Notices for Tryptophanyl tRNA synthetase Antibody (3B2F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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