TSC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2821

Novus Biologicals, part of Bio-Techne
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NB100-2821
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TSC1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QLHIMDYNETHHEH corresponding to C-Terminus according to NP_000359.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_000359.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TSC1 Antibody

Western Blot: TSC1 Antibody [NB100-2821]

Western Blot: TSC1 Antibody [NB100-2821]

Western Blot: TSC1 Antibody [NB100-2821] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821]

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821]

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821] - Staining of paraffin embedded Human Liver. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821]

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821]

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TSC1 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). WB also reported in scientific literature (PMID: 10915759).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TSC1

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GBetaL, which stimulates TOR's kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.

Long Name

Tuberous Sclerosis 1

Alternate Names

Hamartin

Entrez Gene IDs

7248 (Human); 64930 (Mouse)

Gene Symbol

TSC1

UniProt

NP_000359.1 (Human)

Additional TSC1 Products

Product Documents for TSC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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