TSC2 [p Thr1462] Antibody (JE43-81)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32813
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE43-81
Concentration
1 mg/ml
Product Summary for TSC2 [p Thr1462] Antibody (JE43-81)
Immunogen
Synthetic phosphopeptide corresponding to residues around T1462 of human TSC2. (Uniprot: P49815)
Modification
p Thr 1462
Localization
Cytoplasm, Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
201 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TSC2 [p Thr1462] Antibody (JE43-81)
![TSC2 [p Thr1462] Antibody (JE43-81)](https://resources.bio-techne.com/images/products/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813]")
Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813]
Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution.Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate
Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 201 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for TSC2 [p Thr1462] Antibody (JE43-81)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TSC2
Long Name
Tuberous Sclerosis 2
Alternate Names
LAM, TSC4, Tuberin
Gene Symbol
TSC2
Additional TSC2 Products
Product Documents for TSC2 [p Thr1462] Antibody (JE43-81)
Product Specific Notices for TSC2 [p Thr1462] Antibody (JE43-81)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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