Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TSC2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TSC2. The immunogen is located within the first 50 amino acids of TSC2. Amino Acid Squence: TPRPNPRSAEGKQT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TSC2 Antibody - BSA Free

Western Blot: TSC2 AntibodyBSA Free [NBP1-76619]

Western Blot: TSC2 AntibodyBSA Free [NBP1-76619]

Western Blot: TSC2 Antibody [NBP1-76619] - Analysis of TSC2 in L1210 cell lysate with TSC2 antibody at (A) 2 and (B) 4 ug/mL.

Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-76619] -

Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-76619] - L1210 cells with TSC2 antibody at 10 ug/ml.

Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-76619] -

Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-76619] - L1210 cells with TSC2 antibody at 20 ug/mL.
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Applications for TSC2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

UniProt

NP_000539 (Human)

Additional TSC2 Products

Product Documents for TSC2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TSC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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