TSHR Antibody (SPM222)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53224

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM222

Concentration

0.2 mg/ml

View all TSHR Antibodies »

Product Specifications

Immunogen

Recombinant human TSHR protein (Uniprot: P16473)

Localization

Cell Surface & Cytoplasmic

Marker

Thyroid Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54317)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TSHR Antibody (SPM222)

Immunohistochemistry-Paraffin: TSHR Antibody (SPM222) [NBP2-53224]

Immunohistochemistry-Paraffin: TSHR Antibody (SPM222) [NBP2-53224]

Immunohistochemistry-Paraffin: TSHR Antibody (SPM222) [NBP2-53224] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRB Monoclonal Antibody (SPM222).

Applications for TSHR Antibody (SPM222)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TSHR

Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Long Name

Thyroid Stimulating Hormone Receptor

Alternate Names

CHNG1, LGR3, TSH R

Gene Symbol

TSHR

UniProt

P16473

Additional TSHR Products

Product Documents for TSHR Antibody (SPM222)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSHR Antibody (SPM222)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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