TTF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98952

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TTF2 Antibodies »

Product Summary for TTF2 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TTF2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TTF2 Antibody

Western Blot: TTF2 Antibody [NBP2-98952]

Western Blot: TTF2 Antibody [NBP2-98952]

Western Blot: TTF2 Antibody [NBP2-98952] - Anti-TTF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 145 kDa
Immunoprecipitation: TTF2 Antibody [NBP2-98952]

Immunoprecipitation: TTF2 Antibody [NBP2-98952]

Immunoprecipitation: TTF2 Antibody [NBP2-98952] - TTF2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate1 ul anti-TTF2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TTF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 127 kDa. Observed band size: 127 kDa

Applications for TTF2 Antibody

Application
Recommended Usage

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TTF2

TTF2 encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.

Alternate Names

EC 3.6.1, EC 3.6.4.-, F2, HuF2lodestar protein, human factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, transcription termination factor 2, transcription termination factor, RNA polymerase II

Gene Symbol

TTF2

Additional TTF2 Products

Product Documents for TTF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TTF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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