TTLL12 Antibody (OTI7F11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74676
![Flow Cytometry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74676-img0003.jpg "Flow Cytometry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
![Western Blot: TTLL12 Antibody (OTI7F11)Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Western-Blot-NBP2-74676-img0002.jpg "Western Blot: TTLL12 Antibody (OTI7F11)Azide and BSA Free [NBP2-74676]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI7F11
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
74.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TTLL12 Antibody (OTI7F11) - Azide and BSA Free
Western Blot: TTLL12 Antibody (OTI7F11)Azide and BSA Free [NBP2-74676]
Western Blot: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12.![Immunocytochemistry/ Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74676-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
Immunocytochemistry/ Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]
Immunocytochemistry/Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12.![Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74676-img0008.jpg "Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]
Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676] - Staining of paraffin-embedded Ovary tissue using anti-TTLL12 mouse monoclonal antibody.![Western Blot: TTLL12 Antibody (OTI7F11)Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Western-Blot-NBP2-74676-img0001.jpg "Western Blot: TTLL12 Antibody (OTI7F11)Azide and BSA Free [NBP2-74676]")
![Immunocytochemistry/ Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74676-img0004.jpg "Immunocytochemistry/ Immunofluorescence: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
![Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74676-img0006.jpg "Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
![Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]](https://resources.bio-techne.com/images/products/TTLL12-Antibody-OTI7F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74676-img0007.jpg "Immunohistochemistry: TTLL12 Antibody (OTI7F11) - Azide and BSA Free [NBP2-74676]")
Applications for TTLL12 Antibody (OTI7F11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TTLL12
Alternate Names
dJ526I14.2, KIAA0153FLJ41795, tubulin tyrosine ligase-like family, member 12, tubulin--tyrosine ligase-like protein 12
Gene Symbol
TTLL12
Additional TTLL12 Products
Product Documents for TTLL12 Antibody (OTI7F11) - Azide and BSA Free
Product Specific Notices for TTLL12 Antibody (OTI7F11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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