TUFM Antibody (CL2245)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36753
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2245
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSI
Epitope
PFLLPVEAVYSVPGR
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TUFM Antibody (CL2245)
Western Blot: TUFM Antibody (CL2245) [NBP2-36753]
Western Blot: TUFM Antibody (CL2245) [NBP2-36753] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MGImmunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]
Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]
Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular epithelium cells.Applications for TUFM Antibody (CL2245)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TUFM
Alternate Names
EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial
Gene Symbol
TUFM
UniProt
Additional TUFM Products
Product Documents for TUFM Antibody (CL2245)
Product Specific Notices for TUFM Antibody (CL2245)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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