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TUFM Antibody (HL2193) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25731

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25731

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2193

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragmemt of human TUFM.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TUFM Antibody (HL2193) - Azide and BSA Free

TUFM Antibody (HL2193) - Azide and BSA Free

Western Blot: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] -

Western Blot: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody [HL2193] (NBP3-25731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TUFM Antibody (HL2193) - Azide and BSA Free

Knockdown Validated: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] -

Knockdown Validated: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody [HL2193] (NBP3-25731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TUFM Antibody (HL2193) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] -

Immunocytochemistry/Immunofluorescence: TUFM Antibody (HL2193) - Azide and BSA Free [NBP3-25731] - TUFM antibody [HL2193] detects TUFM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUFM stained by TUFM antibody [HL2193] (NBP3-25731) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Applications for TUFM Antibody (HL2193) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

Additional TUFM Products

Product Documents for TUFM Antibody (HL2193) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TUFM Antibody (HL2193) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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