TUFM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84890

Novus Biologicals, part of Bio-Techne
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NBP1-84890
NBP1-84890-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TUFM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%). Reactivity reported in scientific literature (PMID: 22749352)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TUFM Antibody

Western Blot: TUFM Antibody [NBP1-84890]

Western Blot: TUFM Antibody [NBP1-84890]

Western Blot: TUFM Antibody [NBP1-84890] - Analysis using Anti-TUFM antibody NBP1-84890 (A) shows similar pattern to independent antibody NBP1-84889 (B).
Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP1-84890]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP1-84890]

Immunocytochemistry/Immunofluorescence: TUFM Antibody [NBP1-84890] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-84890]

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-84890]

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-84890] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
View 5 more images

Applications for TUFM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

Additional TUFM Products

Product Documents for TUFM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TUFM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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