TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43483

Novus Biologicals, part of Bio-Techne
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NBP1-43483-0.025mg
NBP1-43483-0.1mg
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NBP2-00409
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ITEM-4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TWEAK R/TNFRSF12 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is TWEAKR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reviewed Applications

Read 1 review rated 4 using NBP1-43483 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TWEAK R/TNFRSF12

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Alternate Names

CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR

Gene Symbol

TNFRSF12A

Additional TWEAK R/TNFRSF12 Products

Product Documents for TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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