TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00409
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]](https://resources.bio-techne.com/images/products/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0003.jpg "Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # ITEM-4
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody is TWEAKR.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]](https://resources.bio-techne.com/images/products/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0001.jpg "Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]")
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free [NBP2-00409]
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC. Total viable cells were used for analysis.Applications for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in WB and IHC-F was reported in the scientific literature (PMID: 19473660).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TWEAK R/TNFRSF12
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Alternate Names
CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR
Gene Symbol
TNFRSF12A
Additional TWEAK R/TNFRSF12 Products
Product Documents for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free
Product Specific Notices for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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