TWEAK/TNFSF12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76695

Novus Biologicals, part of Bio-Techne
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NBP1-76695-0.025mg
NBP1-76695
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Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TWEAK/TNFSF12 Antibodies »

Product Specifications

Immunogen

Antibody was raised against recombinant human TWEAK/TNFSF12 protein.

Specificity

Human TWEAK/TNFSF12 has 2 isoforms, including isoform 1 (249aa, 27kD) and isoform 2 (330aa, 36kD). Mouse TWEAK/TNFSF12 has one isoform (249aa, 27kD) and Rat TWEAK/TNFSF12 also has one isoform (249aa, 27kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27, 36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TWEAK/TNFSF12 Antibody - BSA Free

Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]

Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.
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Applications for TWEAK/TNFSF12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5-10 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-4 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TWEAK/TNFSF12

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains. Despite its predicted molecular weight of 27kDa, TWEAK may migrate at aberrant positions in SDS-PAGE.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Entrez Gene IDs

8742 (Human)

Gene Symbol

TNFSF12

UniProt

O43508

Additional TWEAK/TNFSF12 Products

Product Documents for TWEAK/TNFSF12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TWEAK/TNFSF12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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