TXNRD2 Antibody (PSH02-27)

Western Blot: TXNRD2 Antibody (PSH02-27) [NBP3-33069]

Western Blot: TXNRD2 Antibody (PSH02-27) [NBP3-33069] - Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (NBP3-33069) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: A431 cell lysate (20 ug/Lane)
Lane 4: K-562 cell lysate (20 ug/Lane)
Lane 5: Mouse heart tissue lysate (40 ug/Lane)
Lane 6: Rat heart tissue lysate (40 ug/Lane)
Lane 7: Mouse liver tissue lysate (40 ug/Lane)
Lane 8: Rat liver tissue lysate (40 ug/Lane)
Lane 9: Human kidney tissue lysate (40 ug/Lane)
Lane 10: Mouse kidney tissue lysate (40 ug/Lane)
Lane 11: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33069) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TXNRD2 Antibody (PSH02-27) [NBP3-33069]

Immunohistochemistry: TXNRD2 Antibody (PSH02-27) [NBP3-33069] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TXNRD2 antibody (NBP3-33069) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.