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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5H4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 107-379 of human PLAU (NP_002649) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: u-Plasminogen Activator/Urokinase Antibody (OTI5H4)Azide and BSA Free [NBP2-74793]

Western Blot: u-Plasminogen Activator/Urokinase Antibody (OTI5H4)Azide and BSA Free [NBP2-74793]

Western Blot: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY u-Plasminogen Activator.
Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793]

Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793]

Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793]

Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793]

Immunohistochemistry: u-Plasminogen Activator/Urokinase Antibody (OTI5H4) - Azide and BSA Free [NBP2-74793] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: u-Plasminogen Activator (uPA)/Urokinase

Urokinase specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Long Name

Urokinase-type Plasminogen Activator

Alternate Names

PLAU, uPA, uPlasminogen Activator, Urokinase

Gene Symbol

PLAU

Additional u-Plasminogen Activator (uPA)/Urokinase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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