u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05160
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # U-16
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Native human uPA
Epitope
Binds to the B-chain of uPA
Specificity
It is specific for human uPA. No reaction with human tissue plasminogen activator (tPA) when tested by ELISA, immunoblotting and enzyme inhibition and no reaction with any other human plasma proteins when tested by immunoblotting. Binds single and two-chain uPA and uPA/PAI-1 complexes. A reaction is seen with LMW-uPA but not with amino terminal fregment (ATF).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05160 can be used in ELISA. (1) WB: NBP1-05160 reacts with non-reduced uPA but not with reduced uPA in Western blotting. IHC: NBP1-05160 is recommended for flowcytometry and immunoflourescence studies and can be used on frozen sections in immunohistochemistry applications.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: u-Plasminogen Activator (uPA)/Urokinase
Long Name
Urokinase-type Plasminogen Activator
Alternate Names
PLAU, uPA, uPlasminogen Activator, Urokinase
Gene Symbol
PLAU
Additional u-Plasminogen Activator (uPA)/Urokinase Products
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Product Documents for u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free
Product Specific Notices for u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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