U2AF2 Antibody (5G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00011338-M03

Novus Biologicals, part of Bio-Techne
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H00011338-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all U2AF2 Antibodies »

Product Specifications

Immunogen

U2AF2 (NP_001012496.1, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSE

Specificity

This product is specific for Human U2AF2 monoclonal antibody (M03), clone 5G8 [Gene ID: 11338].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for U2AF2 Antibody (5G8)

Western Blot: U2AF2 Antibody (5G8) [H00011338-M03]

Western Blot: U2AF2 Antibody (5G8) [H00011338-M03]

Western Blot: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of U2AF2 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03]

Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03]

Immunocytochemistry/Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of monoclonal antibody to U2AF2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: U2AF2 Antibody (5G8) [H00011338-M03] - Detection limit for recombinant GST tagged U2AF2 is 0.3 ng/ml as a capture antibody.

Applications for U2AF2 Antibody (5G8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a partial recombinant U2AF2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: U2AF2

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Alternate Names

hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit

Gene Symbol

U2AF2

UniProt

NP_001012496.1 (Human)

Additional U2AF2 Products

Product Documents for U2AF2 Antibody (5G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for U2AF2 Antibody (5G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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