U2AF2 Antibody (JE36-33)

Western Blot: U2AF2 Antibody (JE36-33) [NBP3-33071]

Western Blot: U2AF2 Antibody (JE36-33) [NBP3-33071] - Western blot analysis of U2AF2 on different lysates with Rabbit anti-U2AF2 antibody (NBP3-33071) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: U2AF2 Antibody (JE36-33) [NBP3-33071]

Immunohistochemistry: U2AF2 Antibody (JE36-33) [NBP3-33071] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-U2AF2 antibody (NBP3-33071) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33071) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.