UACA Antibody (AE-5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11314

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all UACA Antibodies »

Product Specifications

Immunogen

Nuclei of myeloid leukemia biopsy cells

Localization

Nuclear membrane

Marker

Nuclear Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-11313).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UACA Antibody (AE-5) - Azide and BSA Free

Western Blot: UACA Antibody (AE-5)Azide and BSA Free [NBP3-11314]

Western Blot: UACA Antibody (AE-5)Azide and BSA Free [NBP3-11314]

Western Blot: UACA Antibody (AE-5) - Azide and BSA Free [NBP3-11314] - Western Blot of A549 cell lysate.

Applications for UACA Antibody (AE-5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: UACA

UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

Alternate Names

FLJ10128, KIAA1561MGC141969, MGC141967, nuclear membrane binding protein, NUCLING, uveal autoantigen with coiled coil domains and ankyrin repeats, uveal autoantigen with coiled-coil domains and ankyrin repeats

Gene Symbol

UACA

Additional UACA Products

Product Documents for UACA Antibody (AE-5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UACA Antibody (AE-5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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