UBA3 Antibody (1B6-6-5-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33073
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 1B6-6-5-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human UBA3 aa 220-424 / 463. (Uniprot: Q8TBC4)
Localization
Cytoplasm, cytosol, nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UBA3 Antibody (1B6-6-5-R)
Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073]
Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution.Lane 1: 293T cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: HL-60 cell lysate (20 ug/Lane)
Lane 5: Jurkat cell lysate (20 ug/Lane)
Lane 6: C6 cell lysate (20 ug/Lane)
Lane 7: Mouse brain tissue lysate (40 ug/Lane)
Lane 8: Rat brain tissue lysate (40 ug/Lane)
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for UBA3 Antibody (1B6-6-5-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UBA3
Long Name
Ubiquitin-like Modifier Activating Enzyme 3
Alternate Names
UBE1C
Gene Symbol
UBA3
Additional UBA3 Products
Product Documents for UBA3 Antibody (1B6-6-5-R)
Product Specific Notices for UBA3 Antibody (1B6-6-5-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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