UBA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49443

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-49443
NBP2-49443-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBA3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBA3 Antibody

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.
Western Blot: UBA3 Antibody [NBP2-49443]

Western Blot: UBA3 Antibody [NBP2-49443]

Western Blot: UBA3 Antibody [NBP2-49443] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: UBA3 Antibody [NBP2-49443]

Immunocytochemistry/ Immunofluorescence: UBA3 Antibody [NBP2-49443]

Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-49443] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.
View 2 more images

Applications for UBA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBA3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Ubiquitin-like Modifier Activating Enzyme 3

Alternate Names

UBE1C

Gene Symbol

UBA3

Additional UBA3 Products

Product Documents for UBA3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.