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UbcH10/UBE2C Antibody (CPTC-UBE2C-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07504

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CPTC-UBE2C-1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant human full-length UbcH10/UBE2C protein (Uniprot: O00762)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08829)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UbcH10/UBE2C Antibody (CPTC-UBE2C-1)

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (CPTC-UBE2C-1) [NBP3-07504]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (CPTC-UBE2C-1) [NBP3-07504]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (CPTC-UBE2C-1) [NBP3-07504] - Formalin-fixed, paraffin-embedded human brain stained with UbcH10/UBE2C Mouse Monoclonal Antibody (CPTC-UbcH10/UBE2C-1)

Applications for UbcH10/UBE2C Antibody (CPTC-UBE2C-1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UbcH10/UBE2C

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

UBE2C

Gene Symbol

UBE2C

UniProt

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody (CPTC-UBE2C-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UbcH10/UBE2C Antibody (CPTC-UBE2C-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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