UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20107

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-20107AF350

Alexa Fluor 405

NBP3-20107AF405

Alexa Fluor 488

NBP3-20107AF488

Alexa Fluor 532

NBP3-20107AF532

Alexa Fluor 594

NBP3-20107AF594

Alexa Fluor 647

NBP3-20107AF647

Alexa Fluor 700

NBP3-20107AF700

Alexa Fluor 750

NBP3-20107AF750

Biotin

NBP3-20107B

CoraFluor 1

NBP3-20107CL1

DyLight 350

NBP3-20107UV

DyLight 405

NBP3-20107V

DyLight 488

NBP3-20107G

DyLight 550

NBP3-20107R

DyLight 594

NBP3-20107DL594

DyLight 650

NBP3-20107C

DyLight 680

NBP3-20107FR

DyLight 755

NBP3-20107IR

FITC

NBP3-20107F

HRP

NBP3-20107H

Janelia Fluor 525

NBP3-20107JF525

Janelia Fluor 549

NBP3-20107JF549

Janelia Fluor 585

NBP3-20107JF585

Janelia Fluor 635

NBP3-20107JF635

Janelia Fluor 646

NBP3-20107JF646

Janelia Fluor 669

NBP3-20107JF669

mFluor Violet 450 SE

NBP3-20107MFV450

mFluor Violet 500 SE

NBP3-20107MFV500

mFluor Violet 610 SE

NBP3-20107MFV610

PerCP

NBP3-20107PCP

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-41A-1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all UbcH10/UBE2C Antibodies »

Product Specifications

Immunogen

Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This Ab can be used in immunoMRM.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: UbcH10/UBE2C

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

UBE2C

Gene Symbol

UBE2C

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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