UbcH2/UBE2H Antibody (3C4-1A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00007328-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C4-1A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH2/UBE2H Antibodies »

Product Specifications

Immunogen

UBE2H (AAH06277, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Specificity

UBE2H - ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UbcH2/UBE2H Antibody (3C4-1A2)

Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - UBE2H monoclonal antibody (M01), clone 3C4-1A2 Analysis of UBE2H expression in HeLa.
Immunocytochemistry/ Immunofluorescence: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Immunocytochemistry/ Immunofluorescence: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Immunocytochemistry/Immunofluorescence: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - Analysis of monoclonal antibody to UBE2H on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01]

Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - Analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
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Applications for UbcH2/UBE2H Antibody (3C4-1A2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH2/UBE2H

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

E2-14K, E2-20K, UBC8, UBE2H

Entrez Gene IDs

7328 (Human)

Gene Symbol

UBE2H

OMIM

601082 (Human)

UniProt

AAH06277 (Human)

Additional UbcH2/UBE2H Products

Product Documents for UbcH2/UBE2H Antibody (3C4-1A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH2/UBE2H Antibody (3C4-1A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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