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UbcH5a/UBE2D1 Antibody (SR1267)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22574

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22574-100ul
NBP3-22574-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1267

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human UbcH5a/UBE2D1 (Uniprot #: P51668)

Specificity

Detects endogenous levels of total UbcH5a/UBE2D1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH5a/UBE2D1 Antibody (SR1267)

Western Blot: UbcH5a/UBE2D1 Antibody (SR1267) [NBP3-22574] -

Western Blot: UbcH5a/UBE2D1 Antibody (SR1267) [NBP3-22574] - Western blot analysis using NBP3-22574 in Raji cell lysate.

Applications for UbcH5a/UBE2D1 Antibody (SR1267)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH5a/UBE2D1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Alternate Names

E2(17)KB1, UBC4/5, UBC5A, UBE2D1

Gene Symbol

UBE2D1

Additional UbcH5a/UBE2D1 Products

Product Documents for UbcH5a/UBE2D1 Antibody (SR1267)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UbcH5a/UBE2D1 Antibody (SR1267)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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