Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UbcH5b/UBE2D2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2D2 (NP_003330) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free

Western Blot: UbcH5b/UBE2D2 Antibody (OTI2C2)Azide and BSA Free [NBP2-74732]

Western Blot: UbcH5b/UBE2D2 Antibody (OTI2C2)Azide and BSA Free [NBP2-74732]

Western Blot: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2.
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free [NBP2-74732] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 21 more images

Applications for UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH5b/UBE2D2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

PUBC1, UBC5B, UBE2D2

Gene Symbol

UBE2D2

Additional UbcH5b/UBE2D2 Products

Product Documents for UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UbcH5b/UBE2D2 Antibody (OTI2C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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