UbcH5c/UBE2D3 Antibody (4C1-1E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00007323-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C1-1E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH5c/UBE2D3 Antibodies »

Product Specifications

Immunogen

UBE2D3 (AAH03395, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM

Specificity

UBE2D3 - ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UbcH5c/UBE2D3 Antibody (4C1-1E3)

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - UBE2D3 monoclonal antibody (M01), clone 4C1-1E3 Analysis of UBE2D3 expression in Jurkat.
Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - Antibody Reactive Against Recombinant Protein. Western Blot detection against Immunogen (42.28 KDa) .
Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01]

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - Analysis of UBE2D3 expression in transfected 293T cell line by UBE2D3 monoclonal antibody (M01), clone 4C1-1E3.Lane 1: UBE2D3 transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.
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Applications for UbcH5c/UBE2D3 Antibody (4C1-1E3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH5c/UBE2D3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Alternate Names

UBC5C, UBE2D3

Entrez Gene IDs

7323 (Human)

Gene Symbol

UBE2D3

OMIM

602963 (Human)

UniProt

AAH03395 (Human)

Additional UbcH5c/UBE2D3 Products

Product Documents for UbcH5c/UBE2D3 Antibody (4C1-1E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH5c/UBE2D3 Antibody (4C1-1E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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