UbcH6/UBE2E1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78983

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.02 mg/ml

View all UbcH6/UBE2E1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human UBCH6 protein (between residues 1-50) [UniProt P51965]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH6/UBE2E1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UbcH6/UBE2E1 Antibody - BSA Free [NBP1-78983]

Immunocytochemistry/ Immunofluorescence: UbcH6/UBE2E1 Antibody - BSA Free [NBP1-78983]

Immunocytochemistry/Immunofluorescence: UbcH6/UBE2E1 Antibody [NBP1-78983] - UBCH6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: UbcH6/UBE2E1 Antibody - BSA Free [NBP1-78983]

Immunohistochemistry: UbcH6/UBE2E1 Antibody - BSA Free [NBP1-78983]

Immunohistochemistry: UbcH6/UBE2E1 Antibody [NBP1-78983] - Analysis of UBCH6 in mouse intestine using DAB with hematoxylin counterstain.

Applications for UbcH6/UBE2E1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:600

Immunohistochemistry-Paraffin

1:600
Application Notes
This UBCH6 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus and plasma membrane of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.02 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH6/UBE2E1

Ubiquitination is a cellular process by which the cell identifies abnormal or short-lived proteins for degradation. Three classes of enzymes are involved in this process, ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH6 (UBE2E1 or ubiquitin-conjugating enzyme E2E 1) belongs to the E2 class of enzymes. ISG15, an ubiquitin like protein has been shown to covalently modify UBCH6 as well as forming a thoiester intermediate with ubiquitin. ISG15-modified UBCH6 formed a ubiquitin thioester while the unmodified UBCH6 did not which suggests that ISG156 conjugation suppresses UBCH6 E2 enzyme activity. (PMID: 16428300) In Spinalcerebellar Ataxia-1 (SCA1), research has shown that UBCH6 ubiquinates Ataxin-1. The overexpression of UBCH6 also reduced the transcriptional repression activity of ataxin-1. It addition it was found that overexpression of UbcH6 reduced the formation of Ataxin-1 aggregated. By modulating the degradation of ataxin-1, UbcH6 can regulate the transcriptional repression activity of ataxin-1 (PMID: 18519031).

Alternate Names

UBE2E1

Entrez Gene IDs

7324 (Human)

Gene Symbol

UBE2E1

UniProt

P51965 (Human)

Additional UbcH6/UBE2E1 Products

Product Documents for UbcH6/UBE2E1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UbcH6/UBE2E1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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