UBE2F/NCE2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44062

Novus Biologicals, part of Bio-Techne
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NBP1-44062
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all UBE2F Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HLRDKEDFRNKVDD corresponding to C-Terminus according to NP_542409.1.

Epitope

C-Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBE2F/NCE2 Antibody

Western Blot: UBE2F/NCE2 Antibody [NBP1-44062]

Western Blot: UBE2F/NCE2 Antibody [NBP1-44062]

Western Blot: UBE2F/NCE2 Antibody [NBP1-44062] - (0.05ug/ml) Analysis of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UBE2F/NCE2 Antibody [NBP1-44062]

Immunohistochemistry-Paraffin: UBE2F/NCE2 Antibody [NBP1-44062]

Immunohistochemistry-Paraffin: UBE2F/NCE2 Antibody [NBP1-44062] - Staining of Human Placenta shows membrane staining in trophoblasts.

Applications for UBE2F/NCE2 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
WB: Approx. 20 kDa band observed in mouse liver lysates (calculated MW of 21.1 kDa band according to human NP_542409.1 and to mouse NP_080730.1). IHC-P: Human placenta shows membrane staining in trophoblasts-.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2F

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Long Name

Ubiquitin-conjugating Enzyme E2F

Alternate Names

NCE2

Entrez Gene IDs

140739 (Human); 67921 (Mouse); 363284 (Rat)

Gene Symbol

UBE2F

UniProt

NP_542409.1 (Human)

Additional UBE2F Products

Product Documents for UBE2F/NCE2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UBE2F/NCE2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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