Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UBE2G2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2G2(NP_003334) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2G2 Antibody (OTI1B10) - Azide and BSA Free

Western Blot: UBE2G2 Antibody (OTI1B10)Azide and BSA Free [NBP2-74746]

Western Blot: UBE2G2 Antibody (OTI1B10)Azide and BSA Free [NBP2-74746]

Western Blot: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2G2.
Immunocytochemistry/ Immunofluorescence: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746]

Immunocytochemistry/ Immunofluorescence: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746]

Immunocytochemistry/Immunofluorescence: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY UBE2G2.
Immunohistochemistry: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746]

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746]

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) - Azide and BSA Free [NBP2-74746] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for UBE2G2 Antibody (OTI1B10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2G2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Ubiquitin-conjugating Enzyme E2G 2

Alternate Names

UBC7

Gene Symbol

UBE2G2

Additional UBE2G2 Products

Product Documents for UBE2G2 Antibody (OTI1B10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE2G2 Antibody (OTI1B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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