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UBE2J1 Antibody (6A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00051465-M01

Novus Biologicals, part of Bio-Techne
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H00051465-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

UBE2J1 (NP_003329, 9 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGF

Reactivity Notes

Human. Other species not tested.

Specificity

UBE2J1 (6A12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UBE2J1 Antibody (6A12)

Western Blot: UBE2J1 Antibody (6A12) [H00051465-M01]

Western Blot: UBE2J1 Antibody (6A12) [H00051465-M01]

Western Blot: UBE2J1 Antibody (6A12) [H00051465-M01] - UBE2J1 monoclonal antibody (M01), clone 6A12 Analysis of UBE2J1 expression in HeLa.
Sandwich ELISA: UBE2J1 Antibody (6A12) [H00051465-M01] - Detection limit for recombinant GST tagged UBE2J1 is approximately 0.1ng/ml as a capture antibody.

Applications for UBE2J1 Antibody (6A12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2J1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Long Name

Ubiquitin-conjugating Enzyme E2J 1

Alternate Names

CGI-76, HSPC153, NCUBE1, UBC6e

Entrez Gene IDs

51465 (Human)

Gene Symbol

UBE2J1

Additional UBE2J1 Products

Product Documents for UBE2J1 Antibody (6A12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2J1 Antibody (6A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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