UBE2J1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85654

Novus Biologicals, part of Bio-Techne
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NBP1-85654
NBP1-85654-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2J1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2J1 Antibody

Western Blot: UBE2J1 Antibody [NBP1-85654]

Western Blot: UBE2J1 Antibody [NBP1-85654]

Western Blot: UBE2J1 Antibody [NBP1-85654] - Analysis in control (vector only transfected HEK293T lysate) and UBE2J1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654]

Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654]

Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Analysis in human lymph node and skeletal muscle tissues using NBP1-85654 antibody. Corresponding UBE2J1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654]

Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654]

Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
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Applications for UBE2J1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2J1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Long Name

Ubiquitin-conjugating Enzyme E2J 1

Alternate Names

CGI-76, HSPC153, NCUBE1, UBC6e

Gene Symbol

UBE2J1

Additional UBE2J1 Products

Product Documents for UBE2J1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2J1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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