UBE2K/E2-25K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-840

Novus Biologicals, part of Bio-Techne
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NB300-840
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all UBE2K/E2-25K Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SWDVETATELLLSN corresponding to C-Terminus according to NP_005330.1, NP_001104582.1, NP_001104583.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBE2K/E2-25K Antibody

Western Blot: UBE2K/E2-25K Antibody [NB300-840]

Western Blot: UBE2K/E2-25K Antibody [NB300-840]

Western Blot: UBE2K/E2-25K Antibody [NB300-840] - (1.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840]

Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840]

Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6,

Applications for UBE2K/E2-25K Antibody

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in Jurkat lysates (calculated MW of 22.4 kDa band according to NP_005330). IHC-P: Human brain shows pixulate cytoplasm stainng in neurons of the cortex.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2K/E2-25K

HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequent degradation of p53. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Entrez Gene IDs

3093 (Human)

Gene Symbol

UBE2K

UniProt

NP_005330.1 (Human)

Additional UBE2K/E2-25K Products

Product Documents for UBE2K/E2-25K Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2K/E2-25K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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