UBE2K/E2-25K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30093

Novus Biologicals, part of Bio-Techne
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NBP3-30093
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2K/E2-25K Antibodies »

Product Specifications

Immunogen

Between 150 and 200

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2K/E2-25K Antibody

UBE2K/E2-25K Antibody

Immunoprecipitation:UBE2K/E2-25K AntibodyNBP3-30093] -

Immunoprecipitation:UBE2K/E2-25K AntibodyNBP3-30093] -Detection of human E2-25K by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody NBP3-30093 (lot NBP3-30093-1) used for IP at 6 µg/mg lysate. E2-25K was inefficiently immunoprecipitated by rabbit anti-E2-25K antibody BL14896. For blotting immunoprecipitated E2-25K, NBP3-30093 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for UBE2K/E2-25K Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBE2K/E2-25K

Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as E2-25K, HIP2, and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UBE2K share 100% aa sequence identity. Similar to other Ubiquitin-conjugating (E2) enzymes, UBE2K/E2-25K contains a conserved Ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent Ubiquitin binding surface termed the Ubiquitin-associated domain between aa residues 160 and 200. UBE2K/E2-25K mediates the elongation of Lys48-linked poly-Ubiquitin chains.UBE2K/E2-25K catalytic activity can be modulated by the post-translational addition of SUMO at Lys14. Substrates include the Huntingtin protein and the tumor suppressor RB1. UBE2K/E2-25K is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UBE2K/E2-25K is implicated in polyglutamine diseases and Alzheimer’s disease.

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Gene Symbol

UBE2K

Additional UBE2K/E2-25K Products

Product Documents for UBE2K/E2-25K Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2K/E2-25K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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