UBE2N/Ubc13 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55027
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Summary for UBE2N/Ubc13 Antibody
Immunogen
Synthetic peptides corresponding to UBE2N (ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)) The peptide sequence was selected from the middle region of UBE2N.
Peptide sequence VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQW. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for UBE2N/Ubc13 Antibody
![Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]](https://resources.bio-techne.com/images/products/UBE2N-Ubc13-Antibody-Western-Blot-NBP1-55027-img0003.jpg "Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]")
Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]
Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027] - Lanes: 1 : 40 ng HIS-UBE2D1 protein2: 40 ng HIS-UBE2D2 protein3: 40 ng HIS-UBE2D3 protein4: 40 ng HIS-UBE2D4 protein5: 40 ng HIS-UBE2E1 protein6: 40 ng HIS-UBE2E2 protein7: 40 ng HIS-UBE2E3 protein8: 40 ng HIS-UBE2K protein9: 40 ng HIS-UBE2L3 protein10: 40 ng HIS-UBE2N protein11 : 40 ng HIS-UBE2V1 protein12: 40 ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 50,000 Gene name: UBE2N Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.![Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]](https://resources.bio-techne.com/images/products/UBE2N-Ubc13-Antibody-Western-Blot-NBP1-55027-img0001.jpg "Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]")
Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027]
Western Blot: UBE2N/Ubc13 Antibody [NBP1-55027] - HepG2 cell lysate, concentration 1.25ug/ml.Applications for UBE2N/Ubc13 Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UBE2N/Ubc13
Additional UBE2N/Ubc13 Products
Product Documents for UBE2N/Ubc13 Antibody
Product Specific Notices for UBE2N/Ubc13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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