UBE2T Antibody (OTI2F5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02965
![Western Blot: UBE2T Antibody (OTI2F5) [NBP2-02965]](https://resources.bio-techne.com/images/products/UBE2T-Antibody-2F5-Western-Blot-NBP2-02965-img0001.jpg "Western Blot: UBE2T Antibody (OTI2F5) [NBP2-02965]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2F5
Concentration
0.33 mg/ml
Product Summary for UBE2T Antibody (OTI2F5)
Immunogen
Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UBE2T Antibody (OTI2F5)
Western Blot: UBE2T Antibody (OTI2F5) [NBP2-02965]
Western Blot: UBE2T Antibody (2F5) [NBP2-02965] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.
Immunocytochemistry/Immunofluorescence: UBE2T Antibody (2F5) [NBP2-02965] Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T.
Applications for UBE2T Antibody (OTI2F5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.33 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UBE2T
Long Name
Ubiquitin-conjugating Enzyme E2T
Alternate Names
HSPC150, PIG50
Entrez Gene IDs
29089 (Human)
Gene Symbol
UBE2T
Additional UBE2T Products
Product Documents for UBE2T Antibody (OTI2F5)
Product Specific Notices for UBE2T Antibody (OTI2F5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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