Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UBE2T Antibodies »

Product Summary for UBE2T Antibody (OTI2F5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2T Antibody (OTI2F5) - Azide and BSA Free

Western Blot: UBE2T Antibody (OTI2F5)Azide and BSA Free [NBP2-74750]

Western Blot: UBE2T Antibody (OTI2F5)Azide and BSA Free [NBP2-74750]

Western Blot: UBE2T Antibody (OTI2F5) - Azide and BSA Free [NBP2-74750] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.
Immunocytochemistry/ Immunofluorescence: UBE2T Antibody (OTI2F5) - Azide and BSA Free [NBP2-74750]

Immunocytochemistry/ Immunofluorescence: UBE2T Antibody (OTI2F5) - Azide and BSA Free [NBP2-74750]

Immunocytochemistry/Immunofluorescence: UBE2T Antibody (OTI2F5) - Azide and BSA Free [NBP2-74750] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T.

Applications for UBE2T Antibody (OTI2F5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2T

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 (PubMed 16916645)).(supplied by OMIM)

Long Name

Ubiquitin-conjugating Enzyme E2T

Alternate Names

HSPC150, PIG50

Gene Symbol

UBE2T

Additional UBE2T Products

Product Documents for UBE2T Antibody (OTI2F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE2T Antibody (OTI2F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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