UBE3B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92559

Novus Biologicals, part of Bio-Techne
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NBP1-92559
NBP1-92559-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE3B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE3B Antibody

Immunocytochemistry/ Immunofluorescence: UBE3B Antibody [NBP1-92559]

Immunocytochemistry/ Immunofluorescence: UBE3B Antibody [NBP1-92559]

Immunocytochemistry/Immunofluorescence: UBE3B Antibody [NBP1-92559] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559]

Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559]

Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559] - Staining of human testis shows strong positivity in cytoplasm in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559]

Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559]

Immunohistochemistry-Paraffin: UBE3B Antibody [NBP1-92559] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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Applications for UBE3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29635114).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE3B

UBE3B - ubiquitin protein ligase E3B

Alternate Names

DKFZp586K2123, DKFZp686A1051, EC 6.3.2, EC 6.3.2.-, FLJ45294, MGC131858, MGC78388, ubiquitin protein ligase E3B, ubiquitin-protein ligase E3B

Gene Symbol

UBE3B

Additional UBE3B Products

Product Documents for UBE3B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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