Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all UBE4A Antibodies »

Product Summary for UBE4A Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1016 to 1066 of human Ubiquitination Factor E4A using the numbering given in entry NP_001191006.1 (GeneID 9354).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE4A Antibody

Western Blot: UBE4A Antibody [NBP2-32200]

Western Blot: UBE4A Antibody [NBP2-32200]

Western Blot: UBE4A Antibody [NBP2-32200] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody NBP2-32200 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: UBE4A Antibody [NBP2-32200]

Immunoprecipitation: UBE4A Antibody [NBP2-32200]

Immunoprecipitation: UBE4A Antibody [NBP2-32200] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UBE4A antibody NBP2-32200 used for IP at 6 ug per reaction. For blotting immunoprecipitated UBE4A, NBP2-32200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for UBE4A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBE4A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Alternate Names

E4MGC133315, KIAA0126ubiquitination factor E4A (homologous to yeast UFD2), ubiquitination factor E4A (UFD2 homolog, yeast), UBOX2, UFD2ubiquitin conjugation factor E4 A

Gene Symbol

UBE4A

UniProt

NP_001191006.1 (Human)

Additional UBE4A Products

Product Documents for UBE4A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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