Ubiquitin Antibody (FK1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11039

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # FK1

Concentration

1 mg/ml

View all Ubiquitin Antibodies »

Product Summary for Ubiquitin Antibody (FK1)

Immunogen

Ubiquitin conjugated lysozyme (Uniprot: P0CG47)

Localization

Cytoplasm, Nucleus

Specificity

Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Ubiquitin Antibody (FK1)

Immunocytochemistry/ Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039]

Immunocytochemistry/ Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039]

Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.

Applications for Ubiquitin Antibody (FK1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Gel filtration

Formulation

10 mM phosphate buffer (pH 7.4), 0.15 M NaCl

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Ubiquitin

Ubiquitin is a highly conserved protein of approximately 8.5kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Alternate Names

UBB

Gene Symbol

RPS27A

Additional Ubiquitin Products

Product Documents for Ubiquitin Antibody (FK1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ubiquitin Antibody (FK1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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